Protein_Domain
SS_ompA

Part:BBa_K257006:Design

Designed by: Guillaume Cambray   Group: iGEM09_Paris   (2009-08-20)

ompA sequence signal


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Amplified from the secretion vector pIN-III-ompA-2 (Ghrayeb et al, 1984) keeping the RBS

  1. functions efficiently to secrete a large amount of the OmpA protein
  2. does not require any modification of the cleavage site during the secretion process, in contrast to the polipoprotein

C-term fusion using RFC23 (silver). We can use this sequence signal with TolRII domain (fusion N term) or TE3 Fusion (Nterm)

This part is Inserted in Topo Vector.

Source

pIN-III-ompA-2

References

Ghrayeb et al 1984 - "Secretion Cloning Vectors in Escherichia coli" The EMBO Journal 3(10):2437-2442